Simultaneous Determination and Validation of Triamterene and Benzthiazide by Zero Order Derivative and First Order Derivative Method in Bulk Drug and Pharmaceutical Formulation

The proposed method involves Zero order derivative and First order derivative spectroscopy method. A novel, simple and rapid UV Spectrophotometric determination method for Simultaneous estimation of Triamterene and Benzthiazide was successfully developed and validated in bulk and pharmaceutical formulation. First method is zero order derivative method where the solutions were scanned in the range from 400-200 nm and the peaks were observed at a λmax of 363 and 283nm for Triamterene and Benzthiazide respectively. Second method First order derivative spectroscopy method, which involved the measurement of absorbances of one drug at zero crossing point of other drug. 385 nm and 308 nm were selected for the estimation of Triamterene and Benzthiazide respectively in bulk drug and formulation. Both the methods showed linearity from 6 – 30 μg/ml and 3 -15 μg/ml for Triamterene and Benzthiazide respectively. Recovery studies showed that the method is accurate. Precision of the proposed methods were found to be within the acceptable limits. Thus the two proposed methods and results were validated according to ICH guidelines. So, the methods can be applied for routine analysis in bulk and pharmaceutical formulation.